Studies on Nucleic Acids During Carcinogenesis

Chromatographic Studies on Nucleic Acids

The nucleic acids are such universal constituents of biological systems and their function is probably so important that it is not surprising that much labour has been spent on the study of their composition. Until very recently there have been no methods of analysis which could be considered more than semiquantitative, and it now seems doubtful whether many published analyses are even approxim...

Structural and Biophysical Studies of Nucleic Acids

Solubility and sedimentation studies on certain pentose nucleic acids.

Previous publications from this laboratory (l-3) have described the composition and some chemical properties of certain specimens of ribonucleic acids and of fractions derived from them. Because of the vagueness presently inherent in the term “nucleic acid,” it is believed that any specimen which is studied for any purpose should be characterized by many properties in order to facilitate compar...

Actinomycin D Effects on Nucleic Acids during Tumor Regression1

Early effects of sublethal doses have been compared in sensitive Ridgway osteogenic sarcoma (ROS) and resistant (DMBA) tumors [designation for a spindle cell sarcoma orig inally induced in AKR mice with 7,12-dimethylbenz(o)anthracene] and in small intestine in mice. Doses of actinomycin which were tumoricidal for the sensitive tumor were also toxic to intestinal crypt cells; DNA synthesis, esti...

Conservation of Nucleic Acids during Bacterial Growth

In experiments of 6 hours duration, no replacement of phosphorus or purine and pyrimidine carbon in DNA, nor flow of these atoms from RNA to DNA, could be detected in rapidly growing cultures of E. coli. The slow replacement that has been demonstrated for many substances in non-proliferating tissues of other organisms, though it may occur also in bacteria, is not greatly accelerated under condi...